{"id":213,"date":"2019-08-13T13:54:00","date_gmt":"2019-08-13T04:54:00","guid":{"rendered":"https:\/\/top-researchers.com\/?p=213"},"modified":"2021-10-20T17:48:39","modified_gmt":"2021-10-20T08:48:39","slug":"crispr-cas9%e3%81%ae%e7%ab%8b%e4%bd%93%e6%a7%8b%e9%80%a0%e3%82%92%e3%82%82%e3%81%a8%e3%81%ab%e3%80%81%e3%82%b2%e3%83%8e%e3%83%a0%e7%b7%a8%e9%9b%86%e3%82%92%e3%81%95%e3%82%89%e3%81%aa%e3%82%8b%e9%ab%98","status":"publish","type":"post","link":"http:\/\/top-researchers.com\/?p=213","title":{"rendered":"CRISPR-Cas9\u306e\u7acb\u4f53\u69cb\u9020\u3092\u3082\u3068\u306b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u3055\u3089\u306a\u308b\u9ad8\u307f\u3078\u3068\u5c0e\u304f\u301c\u897f\u5897\u5f18\u5fd7\u30fb\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662 \u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u51c6\u6559\u6388"},"content":{"rendered":"

\u751f\u547d\u306e\u8a2d\u8a08\u56f3\u3067\u3042\u308b\u30b2\u30ce\u30e0\u60c5\u5831\u3092\u66f8\u304d\u63db\u3048\u308b\u300c\u30b2\u30ce\u30e0\u7de8\u96c6\u300d\u3068\u3044\u3046\u8a00\u8449\u304c\u306b\u308f\u304b\u306b\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u30022013\u5e74\u306b\u5831\u544a\u3055\u308c\u305fCRISPR-Cas9\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306f\u3055\u307e\u3056\u307e\u306a\u5206\u91ce\u306b\u6280\u8853\u9769\u65b0\u3092\u3082\u305f\u3089\u3057\u305f\u3002DNA\u3092\u5207\u65ad\u3059\u308b\u30cf\u30b5\u30df\u5f79\u3067\u3042\u308bCas9\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30ac\u30a4\u30c9RNA\u3001\u30bf\u30fc\u30b2\u30c3\u30c8DNA\u304b\u3089\u306a\u308b\u8907\u5408\u4f53\u306e\u7d50\u6676\u69cb\u9020\u3092\u4e16\u754c\u306b\u3055\u304d\u304c\u3051\u3066\u89e3\u660e\u3057\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u308b\u306e\u304c\u3001\u6771\u4eac\u5927\u5b66 \u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u897f\u5897\u5f18\u5fd7 \u51c6\u6559\u6388\u3060\u3002\u897f\u5897\u51c6\u6559\u6388\u306f\u3001\u7acb\u4f53\u69cb\u9020\u306e\u89e3\u660e\u306b\u304f\u308f\u3048\u3001\u5206\u5b50\u69cb\u9020\u3092\u6539\u5909\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u3055\u3089\u306a\u308b\u9ad8\u307f\u3078\u3068\u5c0e\u3044\u3066\u3044\u308b\u3002\u4eca\u56de\u306f\u7814\u7a76\u306e\u7d4c\u7def\u3084\u5c55\u671b\u306b\u3064\u3044\u3066\u3001\u897f\u5897\u51c6\u6559\u6388\u306b\u8a71\u3092\u4f3a\u3063\u305f\u3002<\/p>\n

\"\"<\/p>\n

\u72ec\u81ea\u306e\u300c\u5408\u7406\u8a2d\u8a08\u300d\u624b\u6cd5\u3067\u5206\u5b50\u3092\u6539\u9020<\/h2>\n

Q:\u307e\u305a\u306f\u3001\u7814\u7a76\u306e\u6982\u8981\u306b\u3064\u3044\u3066\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/h3>\n

CRISPR-Cas9\uff08\u30af\u30ea\u30b9\u30d1\u30fc\u30ad\u30e3\u30b9\u30ca\u30a4\u30f3\uff09\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u5831\u544a\u3055\u308c\u305f\u306e\u304c\u30012013\u5e74\u306e1\u6708\u306e\u3053\u3068\u3067\u3057\u305f\u3002Cas9\u3068\u30ac\u30a4\u30c9RNA\uff08Cas9\u3068\u30ac\u30a4\u30c9RNA\u306e\u8907\u5408\u4f53\u3092CRISPR-Cas9\u3068\u3088\u3076\uff09\u3092\u54fa\u4e73\u985e\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3068\u3001\u30b2\u30ce\u30e0DNA\u306e\u72d9\u3063\u305f\u4f4d\u7f6e\u304c\u5207\u65ad\u3055\u308c\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u304d\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u3055\u308c\u305f\u306e\u3067\u3059\u3002
\u5f53\u6642\u306f\u3001Cas9\u304c\u3069\u3046\u3044\u3046\u304b\u305f\u3061\u3092\u3057\u3066\u3044\u3066\u3001\u3069\u306e\u3088\u3046\u306b\u30ac\u30a4\u30c9RNA\u3068\u7d50\u5408\u3057\u3066\u3001\u6a19\u7684\u3068\u306a\u308bDNA\u3092\u898b\u3064\u3051\u51fa\u3057\u3066\u5207\u308b\u306e\u304b\u3001\u305d\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u307e\u3063\u305f\u304f\u308f\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306f\u3001CRISPR-Cas9\u306e\u767b\u5834\u524d\u304b\u3089\u7814\u7a76\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002ZFN\u3084TALEN\u3068\u3088\u3070\u308c\u308b\u4eba\u5de5\u306eDNA\u5207\u65ad\u9175\u7d20\u3092\u7528\u3044\u3066\u30b2\u30ce\u30e0DNA\u3092\u5207\u308b\u3068\u3001\u305d\u308c\u3092\u304d\u3063\u304b\u3051\u306b\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u304d\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001ZFN\u3084TALEN\u306b\u306f\u3001\u4f7f\u3044\u52dd\u624b\u304c\u3088\u304f\u306a\u3044\u3068\u3044\u3063\u305f\u554f\u984c\u70b9\u304c\u6b8b\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n

2007\u5e74\u3053\u308d\u304b\u3089\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3068\u306f\u307e\u3063\u305f\u304f\u5225\u306e\u7814\u7a76\u5206\u91ce\u306b\u304a\u3044\u3066\u3001\u7d30\u83cc\u304cCRISPR-Cas\u3068\u3088\u3070\u308c\u308b\u751f\u4f53\u9632\u5fa1\u6a5f\u69cb\u3092\u3082\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u30a6\u30a4\u30eb\u30b9\u306b\u611f\u67d3\u3057\u305f\u7d30\u83cc\u306fCRISPR-Cas\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u306eDNA\u3092\u7834\u58ca\u3057\u307e\u3059\u3002CRISPR-Cas\u30b7\u30b9\u30c6\u30e0\u306b\u95a2\u308f\u308bCas\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3044\u308d\u3044\u308d\u3042\u308a\u307e\u3059\u304c\u3001Cas9\u306f\u305d\u306e\u3072\u3068\u3064\u3067\u3001\u30ac\u30a4\u30c9RNA\u3068\u5869\u57fa\u5bfe\u3092\u3064\u304f\u308bDNA\u3092\u898b\u3064\u3051\u51fa\u3057\u3066\u5207\u65ad\u3059\u308b\u306f\u305f\u3089\u304d\u3092\u3082\u3061\u307e\u3059\u30022010\u5e74\u304b\u30892012\u5e74\u306b\u304b\u3051\u3066\u3001Cas9\u306e\u8a73\u7d30\u306a\u6a5f\u80fd\u304c\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u305d\u306e\u3053\u308d\u304b\u3089\u3001ZFN\u3084TALEN\u306e\u4ee3\u308f\u308a\u306bCas9\u304c\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u5229\u7528\u3067\u304d\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u308b\u7814\u7a76\u8005\u304c\u5897\u3048\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

2013\u5e74\u306e1\u6708\u306b\u30d6\u30ed\u30fc\u30c9\u7814\u7a76\u6240\u306eFeng Zhang\u306e\u7814\u7a76\u5ba4\u304b\u3089\u3001Cas9\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u5ba4\u304b\u3089\u3001Cas9\u304c\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u4f7f\u3048\u308b\u3068\u3044\u3046\u3053\u3068\u304c\u76f8\u6b21\u3044\u3067\u5831\u544a\u3055\u308c\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u304c\u7206\u767a\u7684\u306b\u9032\u5c55\u3057\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

2012\u5e74\u306e\u5e74\u672b\u3001Feng\u304b\u3089\u300cCas9\u306e\u69cb\u9020\u89e3\u6790\u3092\u4e00\u7dd2\u306b\u3084\u3089\u306a\u3044\u304b\u300d\u3068\u3044\u3046\u30e1\u30fc\u30eb\u304c\u4e0a\u53f8\u306e\u6fe1\u6728 \u7406 \u6559\u6388\u306b\u5c4a\u304d\u307e\u3057\u305f\u3002Feng\u306f\u30b9\u30bf\u30f3\u30d5\u30a9\u30fc\u30c9\u5927\u5b66\u3067\u5149\u907a\u4f1d\u5b66\u3092\u7814\u7a76\u3057\u5b66\u4f4d\u3092\u3068\u3063\u305f\u306e\u3067\u3059\u304c\u3001\u305d\u306e\u5f53\u6642\u3001\u30c1\u30e3\u30cd\u30eb\u30ed\u30c9\u30d7\u30b7\u30f3\u3068\u3044\u3046\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u89e3\u6790\u306b\u95a2\u3057\u3066\u6fe1\u6728\u7814\u7a76\u5ba4\u3068\u5171\u540c\u7814\u7a76\u3092\u884c\u3063\u305f\u3053\u3068\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u305d\u306e\u3088\u3046\u306a\u7e01\u304c\u3042\u3063\u305f\u305f\u3081\u3001Cas9\u306e\u5171\u540c\u7814\u7a76\u306e\u304a\u8a98\u3044\u306e\u30e1\u30fc\u30eb\u3092\u304f\u308c\u305f\u306e\u3067\u3059\u3002\u50d5\u81ea\u8eab\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u6838\u9178\u306e\u8907\u5408\u4f53\u306e\u69cb\u9020\u89e3\u6790\u306b\u8208\u5473\u304c\u3042\u308a\u3001\u3055\u3089\u306b\u30e9\u30c3\u30ad\u30fc\u306a\u3053\u3068\u306b\u3001\u53d6\u308a\u7d44\u3093\u3067\u3044\u305f\u7814\u7a76\u30c6\u30fc\u30de\u304c\u3061\u3087\u3046\u3069\u8ad6\u6587\u306b\u306a\u308a\u3001\u65b0\u3057\u3044\u7814\u7a76\u30c6\u30fc\u30de\u3092\u6a21\u7d22\u3057\u3066\u3044\u305f\u3068\u3053\u308d\u3067\u3057\u305f\u3002\u307e\u3055\u306b\u7d76\u597d\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3067\u3057\u305f\u3002<\/p>\n

Cas9\u306f\u3068\u3066\u3082\u9762\u767d\u3044\u6a5f\u80fd\u3092\u3082\u3064\u65b0\u898f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u306e\u3067\u3001\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u8005\u304c\u8208\u5473\u3092\u3082\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u3068\u304f\u306b\u30012012\u5e74\u306bCas9\u306e\u6a5f\u80fd\u3092\u5831\u544a\u3057\u305fJennifer Doudna\u306e\u7814\u7a76\u5ba4\u306f\u69cb\u9020\u89e3\u6790\u3082\u5f97\u610f\u3067\u3059\u306e\u3067\u3001\u5c11\u306a\u304f\u3068\u3082\u534a\u5e74\u4ee5\u4e0a\u524d\u304b\u3089\u69cb\u9020\u89e3\u6790\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30ea\u30b9\u30af\u306f\u3042\u308b\u3051\u308c\u3069\u3001Cas9\u306e\u4f5c\u52d5\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3044\u3001\u3068\u601d\u3044\u7814\u7a76\u3092\u306f\u3058\u3081\u307e\u3057\u305f\u3002<\/p>\n

\u300c\u3055\u304d\u304c\u3051\u300d\u3068\u3044\u3046\u82e5\u624b\u7814\u7a76\u8005\u3092\u5bfe\u8c61\u3068\u3057\u305f\u30b0\u30e9\u30f3\u30c8\u304c\u3042\u308b\u306e\u3067\u3059\u304c\u30012012\u5e74\u304b\u30893\u5e74\u9593\u3001\u69cb\u9020\u751f\u547d\u79d1\u5b66\u9818\u57df\u306e\u52df\u96c6\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u7b2c2\u671f\u751f\u3068\u3057\u3066\u63a1\u629e\u3057\u3066\u3044\u305f\u3060\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u9762\u63a5\u306e\u969b\u306b\u306f\u30a2\u30c9\u30d0\u30a4\u30b6\u30fc\u306e\u5148\u751f\u304b\u3089\u300c\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u8005\u3068\u306e\u7af6\u4e89\u304c\u3042\u308b\u306e\u306b\u3001\u3042\u306a\u305f\u306b\u52dd\u3061\u76ee\u306f\u3042\u308b\u306e\u304b\uff1f\u300d\u306a\u3069\u3068\u805e\u304b\u308c\u307e\u3057\u305f\u3002<\/p>\n

2014\u5e74\u306e2\u6708\u3001Cas9\u3001\u30ac\u30a4\u30c9RNA\u3001\u30bf\u30fc\u30b2\u30c3\u30c8DNA\u304b\u3089\u306a\u308b\u8907\u5408\u4f53\u306e\u7d50\u6676\u69cb\u9020\u3092\u4e16\u754c\u306b\u3055\u304d\u304c\u3051\u3066\u5831\u544a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3082\u3001\u3055\u307e\u3056\u307e\u306a\u7d30\u83cc\u306b\u7531\u6765\u3059\u308bCas9\u306e\u7d50\u6676\u69cb\u9020\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001Cas9\u306e\u4f5c\u52d5\u6a5f\u69cb\u306e\u5927\u90e8\u5206\u304c\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

Q:\u3053\u3046\u3057\u305f\u306a\u304b\u3001\u897f\u5897\u69d8\u306e\u7814\u7a76\u306b\u306f\u3069\u3046\u3044\u3063\u305f\u72ec\u81ea\u6027\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002<\/h3>\n

Cas9\u306e\u7acb\u4f53\u69cb\u9020\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3060\u3051\u3067\u306f\u306a\u304f\u3001\u65b0\u3057\u3044\u6a5f\u80fd\u3092\u3082\u3064Cas9\u3092\u3064\u304f\u308b\u3053\u3068\u306b\u3082\u6311\u6226\u3057\u3066\u3044\u307e\u3059\u3002Cas9\u306f\u30a2\u30df\u30ce\u9178\u304c\u3064\u306a\u304c\u3063\u3066\u3067\u304d\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u306e\u3067\u3001\u3042\u308b\u5834\u6240\u306e\u30a2\u30df\u30ce\u9178\u306e\u7a2e\u985e\u3092\u5909\u3048\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u305d\u306e\u6027\u8cea\u3092\u5909\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Cas9\u306f\u9023\u7d9a\u3057\u305f2\u3064\u306e\u30b0\u30a2\u30cb\u30f3\u5869\u57fa\uff08PAM\u3068\u3088\u3070\u308c\u308b\uff09\u3092\u76ee\u5370\u306b\u3057\u3066\u6a19\u7684\u3068\u306a\u308bDNA\u3092\u63a2\u3059\u305f\u3081\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u304d\u308b\u907a\u4f1d\u5b50\u306b\u306f\u5236\u9650\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001PAM\u306e\u8fd1\u304f\u306b\u4f4d\u7f6e\u3059\u308b\u30a2\u30df\u30ce\u9178\u3092\u5909\u3048\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u30011\u3064\u306e\u30b0\u30a2\u30cb\u30f3\u5869\u57fa\u3092\u8a8d\u8b58\u3059\u308bCas9\u3092\u3064\u304f\u308a\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u9069\u7528\u7bc4\u56f2\u3092\u62e1\u5f35\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u7570\u306a\u308b\u6a5f\u80fd\u3092\u3082\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3064\u304f\u308b\u306b\u306f\u3044\u304f\u3064\u304b\u306e\u624b\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u7814\u7a76\u8005\u306f\u3001\u30e9\u30f3\u30c0\u30e0\u306b\u5909\u7570\u3092\u5165\u308c\u3066\u65b0\u3057\u3044\u6a5f\u80fd\u3092\u3082\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3068\u3063\u3066\u304f\u308b\u4eba\u5de5\u9032\u5316\u3068\u3088\u3070\u308c\u308b\u624b\u6cd5\u3092\u7528\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n

Cas9\u306b\u95a2\u3057\u3066\u3082\u3001\u7acb\u4f53\u69cb\u9020\u304c\u308f\u304b\u308b\u3068\u3001PAM\u306e\u8a8d\u8b58\u3084DNA\u306e\u5207\u65ad\u306a\u3069\u306e\u6a5f\u80fd\u306b\u91cd\u8981\u306a\u90e8\u5206\u304c\u7279\u5b9a\u3067\u304d\u308b\u308f\u3051\u3067\u3059\u3002
Cas9\u306f\u7d041300\u500b\u306e\u30a2\u30df\u30ce\u9178\u304b\u3089\u306a\u308a\u3001\u30a2\u30df\u30ce\u9178\u306f20\u7a2e\u985e\u3082\u3042\u308b\u306e\u3067\u3001\u3069\u3053\u304c\u91cd\u8981\u304b\u307e\u3063\u305f\u304f\u308f\u304b\u3089\u306a\u3044\u72b6\u614b\u3067\u5909\u7570\u3092\u5165\u308c\u3066\u3044\u304f\u306e\u306f\u5927\u5909\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u7acb\u4f53\u69cb\u9020\u304c\u308f\u304b\u3063\u3066\u3001\u91cd\u8981\u306a\u90e8\u5206\u304c\u3042\u308b\u7a0b\u5ea6\u308f\u304b\u308c\u3070\u3001\u305d\u306e\u90e8\u5206\u306e\u30a2\u30df\u30ce\u9178\u3092\u30e9\u30f3\u30c0\u30e0\u306b\u5909\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u30e9\u30f3\u30c0\u30e0\u306a\u5909\u7570\u3092\u3082\u3064Cas9\u3092\u305f\u304f\u3055\u3093\u3064\u304f\u3063\u3066\u3001\u305d\u306e\u4e2d\u304b\u3089\u76ee\u7684\u306e\u6a5f\u80fd\u3092\u3082\u3064\u3082\u306e\u3092\u3068\u3063\u3066\u304f\u308b\u3068\u3044\u3046\u30a2\u30d7\u30ed\u30fc\u30c1\u304c\u53ef\u80fd\u306b\u306a\u308b\u308f\u3051\u3067\u3059\u3002<\/p>\n

\u72ec\u81ea\u6027\u306e\u89b3\u70b9\u304b\u3089\u3044\u3046\u3068\u3001\u79c1\u305f\u3061\u306f\u30e9\u30f3\u30c0\u30e0\u3067\u306f\u306a\u304f\u3066\u3001\u69cb\u9020\u3092\u898b\u3066\u8a2d\u8a08\u3059\u308b\u3068\u3044\u3046\u300c\u5408\u7406\u8a2d\u8a08\u300d\u306e\u624b\u6cd5\u3092\u3068\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u624b\u6cd5\u3067\u8a2d\u8a08\u3057\u3066\u3044\u308b\u4eba\u305f\u3061\u306f\u4e16\u754c\u7684\u306b\u898b\u3066\u3082\u307b\u3068\u3093\u3069\u3044\u307e\u305b\u3093\u3002<\/p>\n

\u30d1\u30bd\u30b3\u30f3\u306e\u30e2\u30cb\u30bf\u30fc\u4e0a\u3067Cas9\u306e\u5206\u5b50\u69cb\u9020\u3092\u898b\u306a\u304c\u3089\u76ee\u7684\u306e\u6a5f\u80fd\u3092\u3082\u3064\u3088\u3046\u306b\u30a2\u30df\u30ce\u9178\u3092\u3042\u308c\u3053\u308c\u5909\u3048\u3066\u307f\u3066\u3001\u5b9f\u969b\u306b\u5909\u7570\u3092\u5165\u308c\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7cbe\u88fd\u3057\u3001\u305d\u306eDNA\u5207\u65ad\u6d3b\u6027\u3092\u5b9f\u9a13\u7684\u306b\u691c\u8a3c\u3059\u308b\u3001\u3068\u3044\u3046\u30b5\u30a4\u30af\u30eb\u3092\u7e70\u308a\u8fd4\u3057\u3001\u76ee\u7684\u306e\u6a5f\u80fd\u3092\u3082\u3064Cas9\u3092\u3064\u304f\u3063\u3066\u3044\u304d\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u624b\u6cd5\u306f\u4ed6\u3067\u306f\u3042\u307e\u308a\u3084\u3063\u3066\u3044\u307e\u305b\u3093\u3002\u81ea\u5206\u305f\u3061\u3067\u7d50\u6676\u69cb\u9020\u3092\u6c7a\u5b9a\u3057\u3001\u69cb\u9020\u3092\u3088\u304f\u77e5\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u57fa\u76e4\u306b\u306a\u3063\u3066\u3044\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

Q:\u5b9f\u969b\u306e\u7814\u7a76\u306f\u3069\u3046\u3044\u3046\u9032\u3081\u65b9\u306a\u306e\u3067\u3057\u3087\u3046\u304b\u3002<\/h3>\n

\u307e\u3060\u69cb\u9020\u306e\u308f\u304b\u3063\u3066\u3044\u306a\u3044Cas\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u305f\u304f\u3055\u3093\u3042\u308b\u306e\u3067\u3001\u305d\u308c\u3089\u306e\u69cb\u9020\u89e3\u6790\u306b\u3082\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002
\u69cb\u9020\u89e3\u6790\u306e\u624b\u6cd5\u3068\u3057\u3066\u3001\u5f93\u6765\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d50\u6676\u306b\u3057\u3066\u3001X\u7dda\u3092\u5f53\u3066\u3066\u69cb\u9020\u3092\u6c7a\u5b9a\u3059\u308bX\u7dda\u7d50\u6676\u69cb\u9020\u89e3\u6790\u304c\u4e3b\u6d41\u3067\u3057\u305f\u3002<\/p>\n

\u6700\u8fd1\u306f\u3001\u30af\u30e9\u30a4\u30aa\u96fb\u5b50\u9855\u5fae\u93e1\u3092\u7528\u3044\u305f\u5358\u7c92\u5b50\u89e3\u6790\u304c\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u624b\u6cd5\u306f\u4ee5\u524d\u304b\u3089\u3042\u308a\u307e\u3057\u305f\u304c\u3001\u3053\u3053\u6570\u5e74\u306e\u6280\u8853\u9769\u65b0\u306b\u3088\u308a\u5206\u89e3\u80fd\u304c\u5287\u7684\u306b\u5411\u4e0a\u3057\u307e\u3057\u305f\u30022017\u5e74\u306b\u306f\u30ce\u30fc\u30d9\u30eb\u8cde\u3082\u51fa\u3066\u3044\u307e\u3059\u3002<\/p>\n

X\u7dda\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u306f\u3001\u81a8\u5927\u306a\u6761\u4ef6\u3092\u8a66\u3057\u3066\u3088\u3044\u7d50\u6676\u3092\u5f97\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u304c\u96e3\u3057\u3044\u3002\u3055\u3089\u306b\u3001\u3088\u3044\u7d50\u6676\u304b\u3069\u3046\u304b\u306fX\u7dda\u3092\u5f53\u3066\u3066\u307f\u308b\u307e\u3067\u308f\u304b\u308a\u307e\u305b\u3093\u3002\u4e00\u65b9\u3001\u30af\u30e9\u30a4\u30aa\u96fb\u5b50\u9855\u5fae\u93e1\u306e\u5834\u5408\u306f\u3001\u7d50\u6676\u5316\u306e\u5fc5\u8981\u306f\u306a\u304f\u3001\u307e\u305f\u3001\u5c11\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u89e3\u6790\u3067\u304d\u308b\u305f\u3081\u3001\u3053\u308c\u307e\u3067\u69cb\u9020\u89e3\u6790\u3067\u304d\u306a\u304b\u3063\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u304c\u3059\u3054\u3044\u52e2\u3044\u3067\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\u69cb\u9020\u751f\u7269\u5b66\u306e\u7814\u7a76\u624b\u6cd5\u306f\u3053\u306e\u6570\u5e74\u3067\u5927\u304d\u304f\u5909\u308f\u308a\u307e\u3057\u305f\u3002\u65e5\u672c\u306b\u3082\u30af\u30e9\u30a4\u30aa\u96fb\u5b50\u9855\u5fae\u93e1\u304c\u6570\u53f0\u5c0e\u5165\u3055\u308c\u3001\u3053\u30531\u30012\u5e74\u3067\u7814\u7a76\u306e\u9032\u3081\u65b9\u304c\u5927\u304d\u304f\u5909\u308f\u308a\u307e\u3057\u305f\u3002<\/p>\n

Q:\u73fe\u5728\u3001\u7814\u7a76\u5ba4\u306e\u4f53\u5236\u306f\u3069\u3046\u306a\u3063\u3066\u3044\u307e\u3059\u304b\u3002<\/h3>\n

\u73fe\u5728\u306f\u51c6\u6559\u6388\u3068\u3057\u3066\u300110\u4eba\u307b\u3069\u306e\u5b66\u751f\u3068\u4e00\u7dd2\u306b\u7814\u7a76\u3057\u3066\u3044\u307e\u3059\u3002
\u6700\u521d\u3001CRISPR\u3092\u7814\u7a76\u3057\u3066\u3044\u308b\u306e\u306f\u81ea\u5206\u3072\u3068\u308a\u3067\u3057\u305f\u30022015\u5e74\u3053\u308d\u304b\u3089\u5b66\u751f\u3055\u3093\u304c\u52a0\u308f\u308a\u3001\u4e00\u7dd2\u306b\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002<\/p>\n

\u73fe\u5728\u306f\u6388\u696d\u3084\u4ed6\u306e\u4ed5\u4e8b\u3082\u5897\u3048\u3066\u304d\u305f\u3053\u3068\u3082\u3042\u308a\u3001\u307e\u3060\u81ea\u5206\u3067\u3082\u5c11\u3057\u5b9f\u9a13\u3092\u3084\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u307b\u3068\u3093\u3069\u306e\u5b9f\u9a13\u306f\u5b66\u751f\u3055\u3093\u304c\u9032\u3081\u3066\u304f\u308c\u3066\u3044\u307e\u3059\u3002\u5b9f\u9a13\u306f\u597d\u304d\u306a\u306e\u3067\u3001\u3067\u304d\u308b\u3060\u3051\u7d9a\u3051\u305f\u3044\u306e\u3067\u3059\u304c\u3002<\/p>\n

\u8ab2\u984c\u3092\u63b4\u307f\u3001\u6b21\u306a\u308b\u30c6\u30fc\u30de\u3092\u6a21\u7d22\u3059\u308b<\/h2>\n

Q:\u7814\u7a76\u306b\u304a\u3051\u308b\u8ab2\u984c\u3068\u3057\u3066\u611f\u3058\u3066\u3044\u308b\u70b9\u306f\u3069\u3093\u306a\u3053\u3068\u3067\u3057\u3087\u3046\u304b\u3002<\/h3>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u8ab2\u984c\u3068\u3057\u3066\u306f\u3001\u69d8\u3005\u306a\u3068\u3053\u308d\u3067\u8b70\u8ad6\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u502b\u7406\u7684\u306b\u3069\u306e\u3088\u3046\u306b\u30d2\u30c8\u306b\u9069\u7528\u3057\u3066\u3044\u304f\u304b\u3068\u3044\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002
\u3059\u3067\u306b\u4e00\u90e8\u306e\u8fb2\u4f5c\u7269\u306b\u306f\u9069\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u56fd\u306b\u3088\u3063\u3066\u3082\u898f\u5236\u304c\u9055\u3046\u306e\u3067\u3001\u305d\u306e\u3042\u305f\u308a\u306f\u306a\u304b\u306a\u304b\u96e3\u3057\u3044\u3068\u3053\u308d\u3067\u3059\u3002<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u306f\u3001\u9069\u7528\u7bc4\u56f2\u306e\u5236\u9650\u3084\u6a19\u7684\u4ee5\u5916\u3082\u5207\u3063\u3066\u3057\u307e\u3046\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306a\u3069\u306e\u8ab2\u984c\u3082\u6b8b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7c21\u5358\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u4eca\u5f8c\u306f\u305d\u306e\u3088\u3046\u306a\u554f\u984c\u3092\u514b\u670d\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u30c4\u30fc\u30eb\u3092\u958b\u767a\u3067\u304d\u305f\u3089\u3044\u3044\u306a\u3068\u601d\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

Q:\u3053\u306e\u5206\u91ce\u3092\u5fd7\u3059\u5b66\u751f\u306b\u306f\u3001\u3069\u3093\u306a\u3053\u3068\u304c\u5fc5\u8981\u3067\u3057\u3087\u3046\u304b\u3002<\/h3>\n

\u7406\u5b66\u90e8\u751f\u7269\u5316\u5b66\u5c02\u653b\u306e\u5b66\u751f\u306f4\u5e74\u751f\u304b\u3089\u7814\u7a76\u5ba4\u306b\u914d\u5c5e\u306b\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u5927\u5b66\u9662\u304b\u3089\u5165\u3063\u3066\u304f\u308b\u4eba\u3082\u3044\u307e\u3059\u3002
\u7814\u7a76\u30c6\u30fc\u30de\u306f\u3001\u7814\u7a76\u5ba4\u306b\u914d\u5c5e\u3057\u305f\u306e\u3061\u3001\u8a71\u3057\u5408\u3063\u3066\u6c7a\u3081\u307e\u3059\u3002\u5b9f\u9a13\u306e\u624b\u6280\u306f\u3001\u4eba\u306b\u3082\u3088\u308a\u307e\u3059\u304c\u30011\uff5e2\u5e74\u3067\u304b\u306a\u308a\u6163\u308c\u3066\u304d\u307e\u3059\u3002\u624b\u3092\u52d5\u304b\u3057\u3066\u5b9f\u9a13\u3059\u308b\u3053\u3068\u304f\u308f\u3048\u3001\u7814\u7a76\u6210\u679c\u3092\u307e\u3068\u3081\u3066\u767a\u8868\u3057\u305f\u308a\u3001\u8ad6\u6587\u3092\u66f8\u3044\u305f\u308a\u3059\u308b\u3053\u3068\u3082\u91cd\u8981\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u535a\u58eb\u8ab2\u7a0b\u306e\u5b66\u751f\u306f\u81ea\u5206\u3067\u30c6\u30fc\u30de\u3092\u7acb\u6848\u3067\u304d\u308b\u3068\u3053\u308d\u307e\u3067\u3044\u3051\u308b\u3068\u3088\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u4f55\u3092\u3084\u308b\u304b\u3001\u3084\u308b\u610f\u5473\u304c\u3042\u308b\u306e\u304b\u3001\u4ed6\u306e\u7814\u7a76\u8005\u3068\u306e\u7af6\u4e89\u3082\u3042\u308b\u306a\u304b\u3067\u81ea\u5206\u305f\u3061\u306e\u5f37\u307f\u306f\u4f55\u304b\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u3053\u3068\u3092\u8003\u616e\u3057\u3066\u30c6\u30fc\u30de\u3092\u6c7a\u3081\u308b\u306e\u306f\u3001\u7c21\u5358\u3067\u306f\u306a\u3044\u3067\u3059\u304c\u3001\u7814\u7a76\u306b\u304a\u3044\u3066\u3082\u3063\u3068\u3082\u91cd\u8981\u306a\u3053\u3068\u306e\u3072\u3068\u3064\u3060\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u50d5\u81ea\u8eab\u306e\u7d4c\u9a13\u306b\u623b\u308a\u307e\u3059\u304c\u3001Cas9\u306e\u6a5f\u80fd\u306f2012\u5e74\u306e6\u6708\u306b\u5831\u544a\u3055\u308c\u3066\u3044\u305f\u306e\u3067\u30012013\u5e74\u306e1\u6708\u306b\u7814\u7a76\u3092\u958b\u59cb\u3057\u305f\u6642\u70b9\u3067\u3001\u3059\u3067\u306b\u4ed6\u306e\u7814\u7a76\u8005\u306f\u69cb\u9020\u89e3\u6790\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001RNA\u3084DNA\u306e\u7d50\u5408\u3057\u305f\u72b6\u614b\u3067\u306f\u306a\u304f\u3001\u307e\u305aCas9\u306e\u307f\u306e\u72b6\u614b\u306e\u69cb\u9020\u89e3\u6790\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\u3060\u308d\u3046\u3068\u8003\u3048\u307e\u3057\u305f\u3002<\/p>\n

\u30b4\u30fc\u30eb\u304c\u3072\u3068\u3064\u3067\u3042\u308c\u3070\u3001\u5148\u306b\u306f\u3058\u3081\u3066\u3044\u308b\u4eba\u306b\u306f\u52dd\u3066\u306a\u3044\u3067\u3057\u3087\u3046\u3002Cas9\u306f\u3044\u308d\u3044\u308d\u306a\u72b6\u614b\u3092\u3068\u308b\u306e\u3067\u3001\u30b4\u30fc\u30eb\u304c\u3044\u304f\u3064\u3082\u3042\u3063\u305f\u308f\u3051\u3067\u3059\u3002\u6570\u6bb5\u968e\u5148\u306e\u30b4\u30fc\u30eb\u3092\u76ee\u6307\u305b\u3070\u3001\u52dd\u6a5f\u306f\u3042\u308b\u3068\u8003\u3048\u307e\u3057\u305f\u3002\u7814\u7a76\u306f\u52dd\u3061\u8ca0\u3051\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u306a\u304b\u306a\u304b\u305d\u3046\u3044\u3046\u308f\u3051\u306b\u3082\u3044\u304b\u306a\u3044\u306e\u304c\u73fe\u72b6\u3067\u3059\u3002<\/p>\n

\u5b66\u751f\u3055\u3093\u306f\u3068\u3066\u3082\u71b1\u5fc3\u306b\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u5c06\u6765\u306b\u5bfe\u3057\u3066\u60b2\u89b3\u7684\u306b\u306a\u308a\u3059\u304e\u3066\u3044\u308b\u4eba\u304c\u5897\u3048\u3066\u304d\u3066\u3044\u308b\u3088\u3046\u306b\u611f\u3058\u307e\u3059\u3002\u65e5\u672c\u306b\u304a\u3044\u3066\u300c\u535a\u58eb\u300d\u306e\u8a55\u4fa1\u304c\u4f4e\u3044\u3053\u3068\u304c\u4e00\u56e0\u306e\u3088\u3046\u306b\u601d\u3044\u307e\u3059\u3002\u535a\u58eb\u3092\u3068\u3063\u3066\u3082\u8077\u304c\u306a\u3044\u3068\u3044\u3063\u305f\u8a18\u4e8b\u3082\u76ee\u306b\u3057\u307e\u3059\u304c\u3001\u535a\u58eb\u53f7\u3092\u3068\u3063\u3066\u30a2\u30ab\u30c7\u30df\u30c3\u30af\u3084\u4f01\u696d\u3067\u6d3b\u8e8d\u3057\u3066\u3044\u308b\u77e5\u308a\u5408\u3044\u3082\u305f\u304f\u3055\u3093\u3044\u307e\u3059\u3002\u3068\u304f\u306b\u3001\u6d77\u5916\u3067\u30b5\u30a4\u30a8\u30f3\u30b9\u306b\u95a2\u3059\u308b\u4ed5\u4e8b\u3092\u3059\u308b\u306a\u3089\u535a\u58eb\u53f7\u306f\u5fc5\u9808\u306a\u3088\u3046\u3067\u3059\u3002<\/p>\n

1\uff5e2\u5e74\u3084\u3063\u3066\u3082\u7814\u7a76\u6210\u679c\u304c\u51fa\u3066\u3044\u306a\u3044\u3068\u4e0d\u5b89\u306b\u306a\u308b\u5b66\u751f\u3082\u3044\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305f\u3060\u30011\uff5e2\u5e74\u3067\u7814\u7a76\u306b\u5411\u3044\u3066\u3044\u308b\u304b\u3092\u5224\u65ad\u3059\u308b\u306e\u306f\u3001\u5c11\u3057\u65e9\u3059\u304e\u308b\u3088\u3046\u306b\u601d\u3044\u307e\u3059\u3002\u3059\u3050\u306b\u7d50\u679c\u304c\u51fa\u306a\u304f\u3066\u3082\u3001\u3057\u3063\u304b\u308a\u3068\u3057\u305f\u7814\u7a76\u80fd\u529b\u3092\u8eab\u306b\u3064\u3051\u308b\u3053\u3068\u304c\u91cd\u8981\u3060\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u50d5\u306f2007\u5e74\u306b\u535a\u58eb\u53f7\u3092\u3068\u308a\u307e\u3057\u305f\u304c\u3001\u6570\u5e74\u5f8c\u306bCRISPR\u306e\u7814\u7a76\u3092\u3057\u3066\u3044\u308b\u306a\u3093\u3066\u60f3\u50cf\u3082\u3057\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002CRISPR\u306e\u751f\u7406\u7684\u306a\u5f79\u5272\u304c\u5831\u544a\u3055\u308c\u305f\u306e\u304c2007\u5e74\u3067\u3059\u306e\u3067\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u6838\u9178\u306e\u4f5c\u52d5\u6a5f\u69cb\u306b\u8208\u5473\u3092\u3082\u3063\u3066\u7814\u7a76\u3057\u3066\u3044\u305f\u3089\u3053\u3053\u307e\u3067\u304d\u305f\u3001\u3068\u3044\u3046\u611f\u3058\u3067\u3059\u3002\u3044\u308d\u3044\u308d\u3068\u904b\u304c\u3088\u304b\u3063\u305f\u3068\u601d\u3044\u307e\u3059\u3002\u305f\u3060\u3001\u904b\u3060\u3051\u3067\u306a\u304f\u3001\u7814\u7a76\u30c6\u30fc\u30de\u3092\u7acb\u6848\u3057\u3001\u5b9f\u9a13\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\u3092\u884c\u3044\u3001\u8ad6\u6587\u306b\u307e\u3068\u3081\u308b\u3068\u3044\u3046\u4e00\u9023\u306e\u7814\u7a76\u80fd\u529b\u304c\u8eab\u306b\u3064\u3044\u3066\u3044\u305f\u306e\u304c\u3088\u304b\u3063\u305f\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u7814\u7a76\u304c\u597d\u304d\u306a\u3089\u3001\u60b2\u89b3\u7684\u306b\u306a\u308a\u3059\u304e\u305a\u306b\u7814\u7a76\u306b\u6ca1\u982d\u3057\u3066\u307b\u3057\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u5c06\u6765\u3092\u3088\u304f\u8003\u3048\u308b\u3053\u3068\u306f\u5927\u5207\u3067\u3059\u304c\u3001\u3044\u304f\u3089\u8003\u3048\u3066\u3082\u4e88\u60f3\u3067\u304d\u306a\u3044\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u3069\u306e\u7814\u7a76\u5206\u91ce\u3082\u5909\u5316\u304c\u901f\u3044\u306e\u3067\u3001\u4e88\u60f3\u5916\u306e\u5909\u5316\u304c\u7a81\u7136\u8d77\u3053\u308b\u3053\u3068\u3082\u5341\u5206\u306b\u8003\u3048\u3089\u308c\u307e\u3059\u3002CRISPR\u3084\u30af\u30e9\u30a4\u30aa\u96fb\u5b50\u9855\u5fae\u93e1\u304c\u3088\u3044\u4f8b\u3067\u3059\u3002\u65b0\u3057\u3044\u7814\u7a76\u5206\u91ce\u304c\u767b\u5834\u3057\u305f\u3068\u3057\u3066\u3082\u3001\u7814\u7a76\u306e\u57fa\u76e4\u3068\u306a\u308b\u80fd\u529b\u306f\u5909\u308f\u3089\u306a\u3044\u306e\u3067\u3001\u5b66\u751f\u6642\u4ee3\u306f\u3084\u308a\u305f\u3044\u3053\u3068\u3092\u7a81\u304d\u8a70\u3081\u308b\u306e\u304c\u3088\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

Q:\u6700\u5f8c\u306b\u3001\u4eca\u5f8c\u306e\u76ee\u6a19\u306b\u3064\u3044\u3066\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/h3>\n

\u73fe\u5728\u306f\u4e00\u6bb5\u843d\u3057\u305f\u72b6\u614b\u3067\u3082\u3042\u308b\u306e\u3067\u3001\u4f55\u304b\u65b0\u3057\u3044\u3053\u3068\u306b\u6311\u6226\u3057\u305f\u3044\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u81ea\u5206\u306e\u7814\u7a76\u5ba4\u3092\u306f\u3058\u3081\u308b\u3088\u3046\u306a\u6642\u671f\u306b\u306a\u3063\u3066\u304d\u3066\u3044\u308b\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u6570\u5e74\u3067\u74b0\u5883\u3082\u5927\u304d\u304f\u5909\u5316\u3059\u308b\u3088\u3046\u306b\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u5b66\u4f4d\u3092\u53d6\u5f97\u3057\u6fe1\u6728\u7814\u306b\u304d\u306612\u5e74\u3001CRISPR\u306e\u7814\u7a76\u3092\u306f\u3058\u3081\u30666\u5e74\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u9593\u306b\u56fd\u5185\u5916\u306e\u3055\u307e\u3056\u307e\u306a\u4eba\u305f\u3061\u3068\u306e\u3064\u306a\u304c\u308a\u3082\u3067\u304d\u307e\u3057\u305f\u3057\u3001\u60f3\u50cf\u4ee5\u4e0a\u306b\u6210\u9577\u3067\u304d\u305f\u3053\u3068\u306b\u611f\u8b1d\u3057\u3066\u3044\u307e\u3059\u3002\u4eca\u5f8c\u306f\u3053\u308c\u3089\u3092\u6d3b\u304b\u3057\u3066\u9762\u767d\u3044\u3053\u3068\u3092\u3084\u3063\u3066\u3044\u304d\u305f\u3044\u3067\u3059\u3002\uff08\u4e86\uff09<\/p>\n\n\n

\"\"<\/figure><\/div><\/div>

\u897f\u5897 \u5f18\u5fd7<\/h2>

\u306b\u3057\u307e\u3059\u30fb\u3072\u308d\u3057<\/p>

\u6771\u4eac\u5927\u5b66 \u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u51c6\u6559\u6388\u3002<\/p>

2007\u5e74\u6771\u4eac\u5927\u5b66 \u5927\u5b66\u9662\u8fb2\u5b66\u751f\u547d\u79d1\u5b66\u7814\u7a76\u79d1 \u535a\u58eb\u8ab2\u7a0b \u4fee\u4e86\u3002\u535a\u58eb\uff08\u8fb2\u5b66\uff09\u3002<\/p>

\u65e5\u672c\u5b66\u8853\u632f\u8208\u4f1a \u7279\u5225\u7814\u7a76\u54e1PD\uff08\u6771\u4eac\u5927\u5b66\uff09\u3092\u7d4c\u3066\u30012007\u5e7410\u6708\u3088\u308a\u6771\u4eac\u5de5\u696d\u5927\u5b66 \u5927\u5b66\u9662\u751f\u547d\u7406\u5de5\u5b66\u7814\u7a76\u79d1 \u7279\u4efb\u52a9\u6559\u3002<\/p>

\u305d\u306e\u5f8c\u30012008\u5e74\u306b\u306f\u6771\u4eac\u5927\u5b66 \u533b\u79d1\u5b66\u7814\u7a76\u6240\u57fa\u790e\u533b\u79d1\u5b66\u90e8\u9580 \u52a9\u6559\u3068\u306a\u308a\u30012010\u5e74\u304b\u3089\u306f\u6771\u4eac\u5927\u5b66 \u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u7279\u4efb\u52a9\u6559\u30012013\u5e74\u304b\u3089\u306f\u6771\u4eac\u5927\u5b66 \u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u52a9\u6559\u3092\u52d9\u3081\u308b\u3002<\/p>

2019\u5e74\u3088\u308a\u73fe\u8077\u3002<\/p>

\u307e\u305f\u30012013\u5e74\u304b\u30892017\u5e74\u306b\u306f\u3055\u304d\u304c\u3051\u7814\u7a76\u8005\u3082\u517c\u4efb\u3002<\/p><\/div>

    <\/ul><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"

    \u751f\u547d\u306e\u8a2d\u8a08\u56f3\u3067\u3042\u308b\u30b2\u30ce\u30e0\u60c5\u5831\u3092\u66f8\u304d\u63db\u3048\u308b\u300c\u30b2\u30ce\u30e0\u7de8\u96c6\u300d\u3068\u3044\u3046\u8a00\u8449\u304c\u306b\u308f\u304b\u306b\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u30022013\u5e74\u306b\u5831\u544a\u3055\u308c\u305fCRISPR-Cas9\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306f\u3055\u307e\u3056\u307e\u306a\u5206\u91ce\u306b\u6280\u8853\u9769\u65b0\u3092\u3082\u305f\u3089\u3057\u305f\u3002DNA\u3092\u5207\u65ad\u3059\u308b\u30cf\u30b5\u30df\u5f79 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[5,6],"tags":[],"class_list":{"0":"post-213","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-ai-ict-robotics","7":"category-bio-lifescience","8":"entry","9":"has-post-thumbnail"},"featured_image_src":null,"featured_image_src_square":null,"author_info":{"display_name":"Top Researchers","author_link":"http:\/\/top-researchers.com\/?author=1"},"_links":{"self":[{"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/posts\/213","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/top-researchers.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=213"}],"version-history":[{"count":7,"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/posts\/213\/revisions"}],"predecessor-version":[{"id":1756,"href":"http:\/\/top-researchers.com\/index.php?rest_route=\/wp\/v2\/posts\/213\/revisions\/1756"}],"wp:attachment":[{"href":"http:\/\/top-researchers.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=213"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/top-researchers.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=213"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/top-researchers.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=213"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}